Скачать с ютуб Lactate Dehydrogenase Assay | LDH Cytotoxicity Assay | In Vitro Cell Viability By LDH Assay | в хорошем качестве

Lactate Dehydrogenase Assay | LDH Cytotoxicity Assay | In Vitro Cell Viability By LDH Assay |

Lactate dehydrogenase assay When the cell membranes are compromised or u can say damaged, lactate dehydrogenase (LDH), is released into the surrounding extracellular space. Ldh is soluble but stable enzyme present inside every living cell. Now, only way for the LDH to come out of the cell is, when cell membrane integrity is compromised...so this means, the presence of this enzyme in the culture medium can be used as a cell death marker. So lactate dehydrogenase assay is used to measure either... The number of cells via total cytoplasmic lactate dehydrogenase Or membrane integrity as a function of the amount of cytoplasmic LDH released into the medium. So this way, The relative amounts of live and dead cells within the medium, can be quantitated by measuring the amount of released LDH Now to measure the amount of released LDH both colorimetric or fluorometric cytotoxicity assay can be used. In this video we will talk about colorimetric asaay.