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Anti-Streptolysin O (ASO), Test Procedure (positive reaction).

Anti-Streptolysin O (ASO or ASLO) is the antibody made against streptolysin O, an immunogenic, oxygen-labile hemolytic toxin produced by most strains of group A and many strains of groups C and G streptococci. The ASO titer test is a blood test that checks for a strep infection. When you come into contact with harmful bacteria, your body produces antibodies to defend itself against these bacteria and produces antibodies specific to the bacteria they fight. Usually, when you have a strep infection like strep throat, you receive antibiotics that kill the strep bacteria. But some people don’t have any symptoms during a strep infection and may not know they need treatment. When this happens, an untreated infection can lead to future complications. These complications are known as post-streptococcal complications. The letter “O” indicates that this toxin is oxygen labile. The SLO toxin has direct toxic effects on heart tissue. In the course of a streptococcal infection, SLO stimulates the production of specific anti streptolysin (ASO) antibodies, which in-vitro; neutralize the hemolytic properties of the antigen. ASSAY PROTOCOL of Anti-Streptolysin O (ASO or ASLO) 1- Shake the ASO latex reagent gently and add one drop (45 µl) on each circle next to the sample to be tested. 2. Mix well using disposable stirrer spreading the mixture over the whole test area and tilt the slide gently. Agitate for about 2 minutes with a rotator or by hand and observe for the presence or absence of agglutination.