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Detection: RNA-Binding Proteins By RNA Pull-Down In Adipocyte Culture l Protocol Preview

Watch the Full Video at https://www.jove.com/v/54207/detectio.... Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture - a 2 minute Preview of the Experimental Protocol Qianfan Bai, Zhiqiang Bai, Shaohai Xu, Lei Sun Duke-NUS Medical School, Cardiovascular and Metabolic Disorders Program; Institute of Molecular and Cell Biology, Singapore,; Nanyang Technological University, Division of Bioengineering, School of Chemical & Biomedical Engineering; An RNA pull-down protocol is optimized here for detection of interactions between RNA-binding proteins (RBPs) and noncoding as well as coding RNAs. An RNA fragment from androgen receptor (AR) was used as an example to demonstrate how to retrieve its RBP from lystate of primary brown adipocytes. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments