Скачать с ютуб Splicing Activity Complementation by Galectin-3 - U1 snRNP Complex | Protocol Preview в хорошем качестве

Splicing Activity Complementation by Galectin-3 - U1 snRNP Complex | Protocol Preview

Watch the Full Video at https://www.jove.com/v/61990/compleme.... Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads - a 2 minute Preview of the Experimental Protocol Patricia G. Voss, Kevin C. Haudek, Ronald J. Patterson, John L. Wang Michigan State University, Department of Biochemistry and Molecular Biology; Michigan State University, Department of Microbiology and Molecular Genetics; This article describes the experimental procedures for (a) depletion of U1 snRNP from nuclear extracts, with concomitant loss of splicing activity; and (b) reconstitution of splicing activity in the U1-depleted extract by galectin-3 - U1 snRNP particles bound to beads covalently coupled with anti-galectin-3 antibodies. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments